2021-11-12T13:35:20 n13069

Decellularization of porcine liver tissues

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Porcine liver tissues were decellularized to obtain extracellular matrix using chemical detergent based protocols (Protocol I and Protocol II) over a period of 5 days. The extent of decellularization was quantified by assessing the DNA content remaining in the tissues after each day of the chemical treatment using the Picogreen double strand DNA quantification kit. This was normalized to the DNA content of the raw liver tissue to calculate the efficacy of the protocols to remove cellular material from the tissues.

Geographical area of data collection

kmlPolyCoords
153.014003,-27.452963

Publications

Ravichandran, A., Murekatete, B., Moedder, D. et al. Photocrosslinkable liver extracellular matrix hydrogels for the generation of 3D liver microenvironment models. Sci Rep 11, 15566 (2021). https://doi.org/10.1038/s41598-021-94990-z

Research areas

Liver tissue engineering
ECM Hydrogels
Decellularization

Cite this collection

Ravichandran, Akhilandeshwari; Murekatete, Berline; Moedder, Denise; Meinert, Christoph; Bray, Laura; (2021): Decellularization of porcine liver tissues. Queensland University of Technology. (Dataset) https://doi.org/10.25912/RDF_1636688100143

Partner institution

ARC Training Centre for Cell & Tissue Engineering Technologies https://ctet.org.au/
Herston Biofabrication Institute, Metro North Health, Queensland https://metronorth.health.qld.gov.au/herston-biofabrication-institute/

Data file types

.xlsx (Microsoft Excel)

Licence


Creative Commons Attribution 4.0 (CC-BY)
http://creativecommons.org/licenses/by/4.0/

Copyright

© Queensland University of Technology, 2021.

Dates of data collection

From 2020-01-23 to 2020-10-21

Connections

Has association with
Laura Bray  (Researcher)
Has chief investigator

Contacts

Other

Date record created:
2021-09-17T14:11:43
Date record modified:
2021-11-12T13:35:20
Record status:
Published - Open Access