SWATH-MS Proteomic Profiling of Androgen and Anti-androgen Treated LNCaP and C42B Prostate Cancer Cell lines
The current dataset was generated to explore the proteomic alterations stimulated in AR antagonist/anti-androgen (Bicalutamide and Enzalutamide) treated androgen-dependent cell model (LNCaP) in comparison with androgen-independent/castration-resistant cell model (C4-2B). Cells were seeded in 6-well plates using RPMI-1640+5% FBC and were incubated at 37 °C for 48 h. The medium was replaced with androgen-depleted culture medium containing 5% charcoal-striped serum for 48 h. Next, cells were supplemented with androgens: DHT (10 nM DHT), or anti-androgens: BICALUTAMIDE and ENZALUTAMIDE (10 μM) and Ethanol (EtOH: 20%) and incubated at 37 °C for additional 48 has described previously. C4-2B cells were cultured in RPMI-1640+5% FBS.
The SWATH-MS label-free quantification method was used to obtain the total proteome of LNCaP and C42B cell lines. Current dataset provides the DDA and SWATH mass spectrometry files of androgen/anti-androgen treated LNCaP and C42B cell lines.
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