2022-05-31T12:47:59 n21444

A new plant terminator element and its role in small RNA-dependent gene regulation

Viewed: 16

Dataset for the journal article:

de Felippes FF, Shand K and Waterhouse PM (2022) Identification of a Transferrable Terminator Element That Inhibits Small RNA Production and Improves Transgene Expression Levels. Front. Plant Sci. 13:877793. http://dx.doi.org/10.3389/fpls.2022.877793.

The paper describes the identification and characterisation of a new plant element that is involved with the superior performance of the HSP18.2 terminator when driving the expression of transgenes. This element acts by decreasing transcriptional read-through and small RNA generation.

Data has been divided into primary folders labelled to reflect the figures where it is represented. In the case where multiple figure panels are present or multiple datasets were used in conjunction, these folders are further sub-divided and labelled accordingly. Data folders include original images, raw sequencing data, RT-qPCR raw and worked values, GFP quantification data, sRNA blots and RT-PCR gels. 

Geographical area of data collection

text
QUT - Gardens Point Campus, Q Block, Brisbane City, QLD 4000, Australia

Publications

de Felippes FF, Shand K and Waterhouse PM (2022) Identification of a Transferrable Terminator Element That Inhibits Small RNA Production and Improves Transgene Expression Levels. Front. Plant Sci. 13:877793. http://dx.doi.org/10.3389/fpls.2022.877793

Research areas

Other biological sciences
Plant cell and molecular biology
Agricultural molecular engineering of nucleic acids and proteins
Transgenesis
Gene expression (incl. microarray and other genome-wide approaches)
Genome structure and regulation

Cite this collection

Fenselau de Felippes, Felipe; Shand, Kylie; Waterhouse, Peter; (2022): A new plant terminator element and its role in small RNA-dependent gene regulation. Queensland University of Technology. (Collection) https://doi.org/10.25912/RDF_1653963303638

Data file types

The dataset is a combination of .pdf, .xlsx, .jpg and .abi files (requires software for DNA sequencing analysis, e.g. Geneious).

Licence


Creative Commons Attribution 4.0 (CC-BY)
http://creativecommons.org/licenses/by/4.0/

Copyright

© Queensland University of Technology, 2022.

Dates of data collection

From 2018-12-17 to 2022-04-14

Connections

Has association with
Has chief investigator
Peter Waterhouse  (Researcher)

Contacts

Name: Dr Felipe Fenselau de Felippes
Phone: +61 7 3138 7670

Other

Date record created:
2022-05-17T13:19:04
Date record modified:
2022-05-31T12:47:59
Record status:
Published - Open Access