2016-12-20T16:11:46 n4144

Differential expression analysis of genes from the mantle tissue of pearl oyster: Pinctada maxima. Series GSE14303

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The mantle is a thin tissue from which proteins are secreted dictating the mollusk shell construction. As a conserved organ involved in shell formation throughout mollusks, the mantle is an excellent foundation from which to study biomineralization. A P. maxima mantle tissue specific complementary DNA (cDNA) microarray, termed PmaxArray 1.0, has been developed comprising 5000 cDNA transcripts derived from the mantle tissue of P. maxima. This tool has been used to investigate the spatial functional dynamics of the mantle tissue identifying over 2000 PmaxArray 1.0 spots as differentially expressed spatially within this organ. Gene expression profiles observed for these transcripts indicated 5 major spatial functions for the mantle, 3 of which have been putatively attributed to shell formation roles associated with nacre microstructure, calcite prismatic microstructure and periostracum. These transcripts are further examined with in situ expression localization and comparative sequence analyses in reference to potential shell formation roles. This spatial investigation has expedited the elucidation of functions within the dynamic mantle organ, paying particular attention to of shell biomineralization.
The mantle tissue from 9 animals was dissected into 5 separate sections: outer fold (OF), middle fold (MF), inner fold (IF), ventral mantle tissue (VM) and dorsal mantle tissue (DM). Total RNA was extracted from these tissues and pooled across subjects in order to reduce the effect of biological variation; such that 3 individuals were pooled together totaling 3 pooled replicate samples for each tissue. All the biologically pooled tissue types were compared against a common reference in which total RNA from all tissues types and all nine animals was equally pooled. A total of 30 dual channel microarrays hybridizations were performed and analyzed.

Access rights

This data has been published in the Gene Expression Omnibus (GEO) a public functional genomics data repository supporting MIAME-compliant data submissions. Array- and sequence-based data are accepted. Tools are provided to help users query and download experiments and curated gene expression profiles.

Geographical area of data collection

kmlPolyCoords
153.552920,-26.777500 152.452799,-26.777500 152.452799,-28.037280 153.552920,-28.037280 153.552920,-26.777500

Research areas

Gene Expression (incl. Microarray and other genome-wide approaches)
Biomineralization
complementary DNA

Cite this collection

Gardner LD, Elizur A. (2010) Differential expression analysis of genes from the mantle tissue of pearl oyster: Pinctada maxima. Series 14303. [ National Center for Biotechnology Information (NCBI)] http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE14303
Gardner ,Luke; Elizur,A. (2014): Differential expression analysis of genes from the mantle tissue of pearl oyster: Pinctada maxima. Series GSE14303. [Queensland University of Technology]. http://dx.doi.org/10.4225/09/5858cb1577a1a

Copyright

©

Dates of data collection

From 2008-01-01 to 2008-12-31

Contacts

Name: Luke Gardner

Other

Date record created:
2014-07-18T08:40:04
Date record modified:
2016-12-20T16:11:46
Record status:
Published - Open Access