2021-09-01T11:08:42 n9456

Microtissue assembly in Microwell-mesh and implantation in vivo into mice

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This dataset illustrates work and optimisation of a microwell platform named the Microwell-mesh to aggregate precise numbers of cells into arrays of microtissues and the implanting of the Microwell-mesh into NOD-scid IL2γ−/− (NSG) mice to study the microtissue growth. Prostate cancer (PCa) patient-derived xenografts (PDXs) are commonly propagated by serial transplantation of “pieces” of tumour in mice, and using microwell-mesh the cellular composition of pieces becomes standardised.

First, mesh pore size was optimised using microtissues assembled from bone marrow-derived stromal cells, with mesh opening dimensions of 100×100 μm achieving superior microtissue vascularisation relative to mesh with 36×36 μm mesh openings. The optimised Microwell-mesh was used to assemble and implant PCa cell microtissue arrays (microtissues formed from cancer cells are referred to as microtumours) into mice. PCa cells were enriched from three different PDX lines, LuCaP35, LuCaP141, and BM18. 3D microtumours showed greater in vitro viability than 2D cultures, but neither proliferated. Microtumours were successfully established in mice 81% (57 of 70), 67% (4 of 6), 76% (19 of 25) for LuCaP35, LuCaP141, and BM18 PCa cells, respectively. Microtumour growth was tracked using live animal imaging for size or bioluminescence signal. This research data set includes data in relevant folders and formats to each figure in the manuscript which describes the development of the Microwell-mesh as a tool to study microtissues in vitro and in vivo.

The concept of microtissue assembly in the Microwell-mesh, and implantation in vivo may also have utility in implantation of a variety of organ-specific cells that self-assemble into 3D structures, providing an important bridge between in vitro assembly of mini-organs and in vivo implantation, more broadly, a bridge between patients and therapeutic research.

Access rights

This dataset is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.

Geographical area of data collection



Monterosso, Melissa E., Futrega, Kathryn, Lott, William B., Vela, Ian, Williams, Elizabeth D., & Doran, Michael R. (2021) Using the Microwell-mesh to culture microtissues in vitro and as a carrier to implant microtissues in vivo into mice. Scientific Reports, 11, Article number: 5118. http://dx.doi.org/https://eprints.qut.edu.au/209589/

Research areas

Biomedical engineering
Oncology and carcinogenesis

Cite this collection

Monterosso, Melissa; Futrega, Kathryn; Lott, William B; Vela, Ian; Williams, Elizabeth D; Doran, Michael R; (2021): Microtissue assembly in Microwell-mesh and implantation in vivo into mice. Queensland University of Technology. (Dataset) https://doi.org/10.25912/RDF_1624498966255

Related information

Gao, Dong, Vela, Ian, Sboner, Andrea, Iaquinta, Phillip, Karthaus, Wouter, & other. (2014) Organoid cultures derived from patients with advanced prostate cancer. Cell, 159(1), pp. 176-187. https://eprints.qut.edu.au/113863/
McCulloch, Daniel, Opeskin, Kenneth, Thompson, Rik, & Williams, Elizabeth (2005) BM18 : a novel androgen-dependent human prostate cancer xenograft model derived from a bone metastasis. The Prostate, 65(1), pp. 35-43. https://eprints.qut.edu.au/72650/

Partner institution

Queensland Bladder Cancer initiative (QBCI), Brisbane, Australia https://www.linkedin.com/company/queensland-bladder-cancer-initiative-aus-qbci
Translational Research Institute, Brisbane, Australia https://www.tri.edu.au/translational-research-institute-australia
Australian Prostate Cancer Research Centre – Queensland (APCRC-Q) http://www.australianprostatecentre.org/
Department of Urology, Princess Alexandra Hospital, Brisbane, Australia https://metrosouth.health.qld.gov.au/princess-alexandra-hospital

Data file types

Data files consist of.pzfx [Prism], .xlsx [Microsoft Excel], .jpeg, .pptx [Microsoft Powerpoint] and .vsi [OlyVIA1/2].


Creative Commons Attribution 4.0 (CC-BY)


© Queensland University of Technology, 2021.

Dates of data collection

From 2014-09-01 to 2021-03-31


Has association with
Bill Lott  (Researcher)
Elizabeth Williams  (Researcher)
Katarzyna Futrega  (Researcher)
Michael Doran  (Researcher)


Name: Assoc Prof Michael Doran
Phone: +61 7 3443 7348


Date record created:
Date record modified:
Record status:
Published - Open Access