Decellularization of porcine liver tissues
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Porcine liver tissues were decellularized to obtain extracellular matrix using chemical detergent based protocols (Protocol I and Protocol II) over a period of 5 days. The extent of decellularization was quantified by assessing the DNA content remaining in the tissues after each day of the chemical treatment using the Picogreen double strand DNA quantification kit. This was normalized to the DNA content of the raw liver tissue to calculate the efficacy of the protocols to remove cellular material from the tissues.
Geographical area of data collection
kmlPolyCoords
153.014003,-27.452963
Publications
Ravichandran, A., Murekatete, B., Moedder, D. et al. Photocrosslinkable liver extracellular matrix hydrogels for the generation of 3D liver microenvironment models. Sci Rep 11, 15566 (2021).
https://doi.org/10.1038/s41598-021-94990-z
Research areas
Liver tissue engineering
ECM Hydrogels
Decellularization
Cite this collection
Ravichandran, Akhilandeshwari; Murekatete, Berline; Moedder, Denise; Meinert, Christoph; Bray, Laura; (2021): Decellularization of porcine liver tissues. Queensland University of Technology. (Dataset) https://doi.org/10.25912/RDF_1636688100143
Partner institution
ARC Training Centre for Cell & Tissue Engineering Technologies
https://ctet.org.au/
Herston Biofabrication Institute, Metro North Health, Queensland
https://metronorth.health.qld.gov.au/herston-biofabrication-institute/
Data file types
.xlsx (Microsoft Excel)
Licence
Copyright
© Queensland University of Technology, 2021.
Dates of data collection
From 2020-01-23 to 2020-10-21
Contacts
Name: Dr Akhilandeshwari Ravichandran
Phone: +61 469 026 385
Other
Date record created:
2021-09-17T14:11:43
Date record modified:
2021-11-12T13:35:20
Record status:
Published - Open Access