2021-08-13T11:26:56 n3813

COMMD4-mediated chromatin regulation: peptide-mapping and mutagenesis data

Viewed: 31

Genomic stability is critical for normal cellular function and its deregulation is a universal hallmark of cancer. Here, we outline a previously undescribed role of COMMD4 in maintaining genomic stability, by regulation of chromatin remodelling at sites of DNA double-strand breaks. At break-sites, COMMD4 binds to and protects histone H2B from monoubiquitination by RNF20/RNF40. DNA damage-induced phosphorylation of the H2A-H2B heterodimer disrupts the dimer allowing COMMD4 to preferentially bind H2A. Displacement of COMMD4 from H2B allows RNF20/40 to monoubiquitinate H2B and for remodelling of the break-site. Consistent with this critical function, COMMD4-deficient cells show excessive elongation of remodelled chromatin and failure of both non-homologous-end-joining and homologous recombination.

We present peptide-mapping and mutagenesis data for the potential molecular mechanisms governing COMMD4-mediated chromatin regulation at DNA double-strand breaks. 

Geographical area of data collection

kmlPolyCoords
152.270706,-26.984704

Publications

Suraweera, Amila, Gandhi, Neha S., Beard, Sam, Burgess, Joshua T., Croft, Laura V., Bolderson, Emma, Naqi, Ali, Ashton, Nicholas W., Adams, Mark N., Savage, Kienan I., Zhang, Shu Dong, O’Byrne, Kenneth J., & Richard, Derek J. (2021) COMMD4 functions with the histone H2A-H2B dimer for the timely repair of DNA double-strand breaks. Communications Biology, 4, Article number: 484. https://eprints.qut.edu.au/210226/

Research areas

Medicinal and biomolecular chemistry
Biochemistry and cell biology
Oncology and carcinogenesis

Cite this collection

Suraweera, Amila; Gandhi, Neha S.; Beard, Sam; Burgess, Joshua T.; Croft, Laura V.; Bolderson, Emma; Naqi, Ali; Ashton, Nicholas W.; Adams, Mark N.; Savage, Kienan I.; Zhang, Shu-Dong; O'Byrne, Kenneth J.; Richard, Derek J.; (2021): COMMD4-mediated chromatin regulation: peptide-mapping and mutagenesis data. Queensland University of Technology. (Dataset) https://doi.org/10.25912/RDF_1627003675622

Partner institution

Data file types

Data files are in .pdf and .xls format.

Licence


Creative Commons Attribution 4.0 (CC-BY)
http://creativecommons.org/licenses/by/4.0/

Copyright

© Queensland University of Technology, 2021.

Dates of data collection

From 2015-01-02 to 2020-06-01

Connections

Has association with
Derek Richard  (Researcher)
Emma Bolderson  (Researcher)
Ken O'Byrne  (Researcher)
Laura Croft  (Researcher)
Mark Adams  (Researcher)
Neha S. Gandhi  (Researcher)
Has chief investigator
Amila Suraweera  (Researcher)

Contacts

Name: Dr Amila Surawera
Phone: + 61 3443 7315

Other

Date record created:
2021-06-18T14:58:22
Date record modified:
2021-08-13T11:26:56
Record status:
Published - Open Access